Breakthrough Technologies Activation Tagging in Arabidopsis
نویسندگان
چکیده
Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and Howard Hughes Medical Institute (J.C.), The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037; Department of Biology, University of California at San Diego, La Jolla, California 92093–0116 (C.Ferrándiz, S.S., M.F.Y.); and Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402 (I.K., Y.X., R.A.D., M.J.H.)
منابع مشابه
Breakthrough Technologies High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794–5215 (G.-W.T., B.P., C.D.K., J.L., V.C.); Watson School of Biological Sciences (C.D.K.) and Cold Spring Harbor Laboratory (A.M., D.J.), Cold Spring Harbor, New York 11724; Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, California...
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Activation tagging is a powerful tool to identify new mutants and to obtain information about possible biological functions of the overexpressed genes. The quadruple cauliflower mosaic virus (CaMV) 35S enhancer fragment is a strong enhancer, which is most commonly used for this purpose. However, the constitutive nature of this enhancer may generate lethal mutations or aberrations in different p...
متن کاملActivation tagging in Arabidopsis.
Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have been used to generate several tens of thousands of transformed plants. From these, over 30 dominant ...
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The plant-pathogen interaction is a multifactor process that may lead to resistance or susceptible responses of plant to pathogens. During the arms race between plant and pathogens, various biochemical, molecular and physiological events are triggered in plant cells such as ROS signaling, hormone activation and gene expression reprogramming. In plants, microRNAs (miRNAs) are key post-transcript...
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To monitor the expression of T-DNA-tagged plant genes in vivo, a collection of 20,261 transgenic lines of Arabidopsis (Columbia-0) were generated with the promoter trap vector pTluc, which carries a promoterless firefly luc (luciferase) reporter gene linked to the right T-DNA border. By detection of bioluminescence in 3-week-old seedlings, 753 lines were identified showing constitutive, organ-s...
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